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UCI Chem 128 Introduction to Chemical Biology (Winter 2013)
Lec 13. Introduction to Chemical Biology -- Protein Function and Enzymes
View the complete course: ../courses/chem_128_introduction_to_chemical_biology.html
Instructor: Gregory Weiss, Ph.D.

License: Creative Commons BY-NC-SA
Terms of Use: ../info.
More courses at http://ocw.uci.edu

Description: This video is part of a 18-lecture undergraduate-level course titled "Introduction to Chemical Biology" taught at UC Irvine by Professor Gregory Weiss. Introduction to the basic principles of chemical biology: structures and reactivity; chemical mechanisms of enzyme catalysis; chemistry of signaling, biosynthesis, and metabolic pathways.
Introduction to Chemical Biology (Chem 128) is part of OpenChem: ../openchem

Recorded February 21, 2013.

Index of Topics:
0:00:29 - Week 7 Overview
0:00:41 - Our Story to Date...
0:03:20 - Repeat Proteins
0:05:16 - Equilibrium Constants to Describe the Strengths of Non-Covalent Interactions
0:06:25 - Enzymatic Catalysts = Catalytic Receptors
0:06:55 - Enzymes Work By Stabilizing & Thus Lowering the Energy of Transition States
0:07:03 - Michaelis Constant for Measuring Catalysis
0:10:53 - Typical Km Values
0:12:47 - Typical Kcat Values
0:15:30 - The Perfect Enzyme
0:18:02 - Systematic Examining Enzymes
0:21:29 - Kinases: Phosphorylation of Ser/Thr or Tyr
0:22:41 - Can Inhibit Enzymes by Substrate Mimicry
0:24:24 - The Kinase Active Site
0:26:03 - The Mechanism of Kinase Catalysis
0:28:06 - Kinase Activity is Tightly Regulated
0:31:17 - Phosphorylation to Activate Kinase
0:34:19 - Why Study Single Molecules?
0:37:44 - How to Follow Enzymatic Catalysis with Single Walled Carbon Nanotubes
0:41:34 - Single Molecule Bioelectronics
0:42:29 - A Single Enzyme in the Nanocircuit
0:44:24 - Before and After Enzyme Attachment
0:46:29 - Watching cAMP-Dependent Protein Kinase A
0:49:18 - Further Generalization: Protein A Kinase
0:55:55 - Lysozyme as a Model Enzyme for Glycoside Hydrolysis
0:57:37 - Mechanism of Lysozyme (Example of a Hydrolase)
1:02:07 - Comparing Single Molecule Signals: Bioelectronic vs. FRET
1:02:14 - Watching a Single Enzyme-Electronically
1:02:42 - Lysozyme Exhibits Two Types of Binary Switching
1:04:23 - Individual Lysozymes on Nanotubes Confirms Previous Studies
1:04:29 - Lysozyme Processively Transits Through Peptide Cross-Links in the Substrate
1:05:29 - Lysozyme Processively...Slide 2
1:08:22 - Proteases Cleave Amide Bonds
1:09:19 - Mutations In Protease-Based Pathways Can Have Dire Consequences
1:09:41 - Apoptosis is Regulated By a Cascade of Protease Reactions
1:10:06 - Cysteine-Based Proteases
1:10:46 - Mechanism for Cysteine-Based Protease
1:12:21 - Serine-Based Proteases
1:12:30 - Serine Proteases
1:12:32 - Zinc Proteases
1:12:37 - Regulation of Proteases Through Pro-Enzymes
1:13:38 - Inhibiting Protease Reversibility
1:14:35 - Protein Based Inhibition of Proteases

Required attribution: Weiss, Gregory Introduction to Chemical Biology 128 (UCI OpenCourseWare: University of California, Irvine),  ../courses/chem_128_introduction_to_chemical_biology.html. [Access date]. License: Creative Commons Attribution-ShareAlike 3.0 United States License.